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黄锦荣1, 张凤2, 谢金兰2, 张冬生2, 朱昔娇2, 唐国涛2, 范秀琼2, 范剑明2
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1.梅州市农林科学院林业研究所;2.梅州市林业科学研究所
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摘要: |
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以五指毛桃 Ficus hirta 优良植株健壮的带顶芽嫩枝为外植体，进行组织培养育苗研究，结果表明：最合适的外植体灭菌方法是1 g·L-1 的升汞溶液灭菌14 min；合适的芽诱导培养基为MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1+ 蔗糖 30 g·L-1+卡拉胶6.5 g·L-1，诱导率为40%；最适的增殖培养基为MS+6-BA 1.0 mg·L-1+IBA 0.3 mg·L-1+ 蔗糖30 g·L-1+卡拉胶6.5 g·L-1，增殖倍数为5.43；最佳的生根培养基为1/4MS+IBA 1.5 mg·L-1+NAA 0.3 mg·L-1+蔗糖20 g·L-1+卡拉胶6.5 g·L-1+活性碳0.5 g·L-1，生根率为96%；最佳的瓶苗移栽基质为轻型基质，存活率为98.15%。
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关键词:
五指毛桃；组织培养；外植体；芽诱导；生根
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DOI：
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分类号：
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基金项目：广东省地方标准制订项目（2019-DB-28）；广东省科技厅项目（2019A0103004）；梅州市科技局项目（2019B0203002）； 广东省科技厅项目（2020A0104007） |
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Study on Tissue Culture of Ficus hirta |
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Institute of Forestry, Meizhou Academy of Agriculture and Forestry Sciences
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Abstract: |
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The tissue culture of Ficus hirta was studied, selecting the robust shoots with apical buds of the
as experiment material. The results showed that in this study the most suitable sterilization treatment for shoots is soaking in 1 g·L-1 mercury solution for 14 minutes. The most suitable bud induction medium is MS+6-BA 1.0 mg·L-1+IBA0.1 mg·L-1+sucrose 30 g·L-1+carrageenan 6.5 g· L-1, which induce rate is 40%. The most suitable proliferation medium is MS+6-BA 1.0 mg·L-1+IBA0.3 mg · L-1+sucrose 30 g·L-1+carrageenan 6.5 g·L-1, which the multiplication index is 5.43. The best rooting medium is 1/4MS+IBA1.5 mg·L-1+NAA0.3 mg·L-1+sucrose 20 g·L-1+carrageenan 6.5 g·L-1 + activated carbon 0.5 g·L-1, which the rooting rate is 96%. The best seedling transplanting medium is a light substrate with a survival rate of 98.15%.
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Key words:
Ficus hirta; tissue culture; explants; bud induction; rooting
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